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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65150-1-Ig (same clone as 65150-1-PBS)

    FC experiment of human PBMCs using 65150-1-Ig (same clone as 65150-1-PBS)

    1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD45RA and 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65150-1-Ig (same clone as 65150-1-PBS)

    FC experiment of human PBMCs using 65150-1-Ig (same clone as 65150-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 65150-1-Ig (same clone as 65150-1-PBS)

    IF Staining of human colon cancer using 65150-1-Ig (same clone as 65150-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CD45RO antibody (65150-1-Ig, Clone: UCHL1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 65150-1-Ig (same clone as 65150-1-PBS)

    IHC staining of human tonsillitis using 65150-1-Ig (same clone as 65150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD45 antibody included
  • IHC staining of human tonsillitis using 65150-1-Ig (same clone as 65150-1-PBS)

    IHC staining of human tonsillitis using 65150-1-Ig (same clone as 65150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD45 antibody included
  • IHC staining of human colon cancer using 65150-1-Ig (same clone as 65150-1-PBS)

    IHC staining of human colon cancer using 65150-1-Ig (same clone as 65150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD45 antibody included

Anti-Human CD45RO (UCHL1), PBS Only

CD45RO Monoclonal Antibody for IHC, IF-P, FC
Cat No. 65150-1-PBS
CloneNo. UCHL1

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

IHC, IF-P, FC

Synonyms

CD45, B220, LCA, L CA, GP180

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

产品信息

65150-1-PBS targets CD45RO in IHC, IF-P, FC applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, FC Application Description
Tested Reactivity human
Immunogen IL-2 dependent T cell line, CA1 种属同源性预测
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms CD45, B220, LCA, L CA, GP180
GenBank Accession NumberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2881476
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08575
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).