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验证数据展示

  • FC experiment of human PBMCs using 65150-1-Ig (same clone as 65150-1-PBS)

    FC experiment of human PBMCs using 65150-1-Ig (same clone as 65150-1-PBS)

    1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD45RA and 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65150-1-Ig (same clone as 65150-1-PBS)

    FC experiment of human PBMCs using 65150-1-Ig (same clone as 65150-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 65150-1-Ig (same clone as 65150-1-PBS)

    IF Staining of human colon cancer using 65150-1-Ig (same clone as 65150-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CD45RO antibody (65150-1-Ig, Clone: UCHL1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 65150-1-Ig (same clone as 65150-1-PBS)

    IHC staining of human tonsillitis using 65150-1-Ig (same clone as 65150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD45 antibody included
  • IHC staining of human tonsillitis using 65150-1-Ig (same clone as 65150-1-PBS)

    IHC staining of human tonsillitis using 65150-1-Ig (same clone as 65150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD45 antibody included
  • IHC staining of human colon cancer using 65150-1-Ig (same clone as 65150-1-PBS)

    IHC staining of human colon cancer using 65150-1-Ig (same clone as 65150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65150-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD45 antibody included

Anti-Human CD45RO (UCHL1), PBS Only

CD45RO Monoclonal Antibody for IHC, IF-P, FC
Cat No. 65150-1-PBS

产品说明书

CloneNo. UCHL1

宿主/亚型

Mouse / IgG2a, kappa

种属反应性

human

应用

IHC, IF-P, FC

别名

CD45, B220, LCA, L CA, GP180

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

产品信息

65150-1-PBS targets CD45RO in IHC, IF-P, FC applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, FC Application Description
经测试反应性 human
免疫原 IL-2 dependent T cell line, CA1 种属同源性预测
宿主/亚型 Mouse / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 CD45, B220, LCA, L CA, GP180
GenBank蛋白编号BC014239
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2881476
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08575
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).