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CD46 Polyclonal antibody, PBS Only

CD46 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28936-1-PBS

Print datasheet

Synonyms

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

验证数据展示

  • WB analysis using 28936-1-AP (same clone as 28936-1-PBS)

    WB analysis using 28936-1-AP (same clone as 28936-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

    IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD46 antibody included
  • IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

    IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD46 antibody included
  • IF Staining of HeLa using 28936-1-AP (same clone as 28936-1-PBS)

    IF Staining of HeLa using 28936-1-AP (same clone as 28936-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CD46 antibody (28936-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD46 Ready-To-Use IHC Kit. CD46 primary antibody included.

产品信息

28936-1-PBS targets CD46 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD46 fusion protein Ag30376 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD46 molecule, complement regulatory protein
Synonyms TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP
Calculated Molecular Weight 384 aa, 43 kDa
Observed Molecular Weight50-70 kDa
GenBank Accession NumberBC030594
Gene Symbol CD46
Gene ID (NCBI) 4179
RRIDAB_2881233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15529
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).