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CD48 Polyclonal antibody

CD48 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27519-1-AP

Print datasheet

Synonyms

hCD48, CD48 molecule, CD48 antigen, B-lymphocyte activation marker BLAST-1, BLAST1

验证数据展示

  • WB analysis of Raji using 27519-1-AP

    WB analysis of Raji using 27519-1-AP

    Raji cells and Ramos cells were subjected to SDS PAGE followed by western blot with 27519-1-AP (CD48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27519-1-AP

    IHC staining of human tonsillitis using 27519-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27519-1-AP (CD48 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27519-1-AP

    IHC staining of human tonsillitis using 27519-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27519-1-AP (CD48 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRaji cells, Daudi cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27519-1-AP targets CD48 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen CD48 fusion protein Ag26536 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD48 molecule
Synonyms hCD48, CD48 molecule, CD48 antigen, B-lymphocyte activation marker BLAST-1, BLAST1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC016182
Gene Symbol CD48
Gene ID (NCBI) 962
RRIDAB_2880897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09326
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.

实验方案

Product Specific Protocols
WB protocol for CD48 antibody 27519-1-APDownload protocol
IHC protocol for CD48 antibody 27519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols