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验证数据展示

  • WB analysis using 31945-1-AP

    WB analysis using 31945-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31945-1-AP (CD48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 31945-1-AP

    IHC staining of human tonsillitis using 31945-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31945-1-AP (CD48 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 31945-1-AP

    IHC staining of human tonsillitis using 31945-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31945-1-AP (CD48 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD48 Polyclonal antibody

CD48 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31945-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

hCD48, CD48 molecule, CD48 antigen, B-lymphocyte activation marker BLAST-1, BLAST1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, Jurkat cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31945-1-AP targets CD48 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD48 molecule
别名 hCD48, CD48 molecule, CD48 antigen, B-lymphocyte activation marker BLAST-1, BLAST1
计算分子量 43 kDa
观测分子量45-55 kDa
GenBank蛋白编号BC016182
基因名称 CD48
Gene ID (NCBI) 962
RRIDAB_3670153
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP09326
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.

实验方案

Product Specific Protocols
WB protocol for CD48 antibody 31945-1-APDownload protocol
IHC protocol for CD48 antibody 31945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols