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CoraLite®594 Anti-Mouse CD49b (DX5)

CD49b Monoclonal Antibody for FC

Host / Isotype

Rat / IgM, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

DX5

Cat no : CL594-65065

Print datasheet

Synonyms

CD49B, DX5, integrin alpha 2, Itga2

验证数据展示

  • FC experiment of mouse splenocytes using CL594-65065

    FC experiment of mouse splenocytes using CL594-65065

    1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse NK1.1 (CD161) (APC-65138, Clone: PK136) and 5.00 ul CoraLite®594-conjugated Anti-Mouse CD49b (CL594-65065, Clone: DX5). Cells were not fixed.

  • FC experiment of mouse splenocytes using CL594-65065

    FC experiment of mouse splenocytes using CL594-65065

    1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse NK1.1 (CD161) (APC-65138, Clone: PK136) and CoraLite®594-conjugated Rat IgM isotype control antibody. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65065 targets CD49b in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen NK cells isolated from C57BL/6 mice 种属同源性预测
Host / Isotype Rat / IgM, kappa
Class Monoclonal
Type Antibody
Full Name integrin alpha 2
Synonyms CD49B, DX5, integrin alpha 2, Itga2
GenBank Accession NumberBC065139
Gene Symbol CD49b
Gene ID (NCBI) 16398
RRIDAB_2919931
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ62469
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.

实验方案

Product Specific Protocols
FC protocol for CL594 CD49b antibody CL594-65065Download protocol
Standard Protocols
Click here to view our Standard Protocols