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验证数据展示

  • WB analysis using 31502-1-AP

    WB analysis using 31502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31502-1-AP (CD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendicitis using 31502-1-AP

    IHC staining of human appendicitis using 31502-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 31502-1-AP (CD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 31502-1-AP

    IHC staining of human appendicitis using 31502-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 31502-1-AP (CD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD5 Polyclonal antibody

CD5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31502-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MJ cells, MOLT-4 cells
Positive IHC detected inhuman appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31502-1-AP targets CD5 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD5 molecule
别名 CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1
计算分子量55 kDa
观测分子量60 kDa
GenBank蛋白编号NM_014207.4
基因名称 CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
RRIDAB_3670009
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP06127
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
WB protocol for CD5 antibody 31502-1-APDownload protocol
IHC protocol for CD5 antibody 31502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols