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Anti-Human CD5 (UCHT2) Rabbit IgG Recombinant Antibody, PBS Only

CD5 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

UCHT2

Cat no : 65513-1-PBS

Print datasheet

Synonyms

T-cell surface glycoprotein CD5, T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1

验证数据展示

  • FC experiment of human PBMCs using 65513-1-PBS

    FC experiment of human PBMCs using 65513-1-PBS

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65513-1-RR (same clone as 65513-1-PBS)

    FC experiment of human PBMCs using 65513-1-RR (same clone as 65513-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65513-1-RR (same clone as 65513-1-PBS)

    FC experiment of human PBMCs using 65513-1-RR (same clone as 65513-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65513-1-PBS

    FC experiment of human PBMCs using 65513-1-PBS

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65513-1-PBS targets CD5 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD5 molecule
Synonyms T-cell surface glycoprotein CD5, T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1
Calculated Molecular Weight 495 aa, 55 kDa
GenBank Accession NumberBC027901
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
FC protocol for CD5 antibody 65513-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols