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Anti-Human CD5 (L17F12) Mouse IgG1 Recombinant Antibody, PBS Only

CD5 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

L17F12

Cat no : 65530-1-PBS

Print datasheet

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

验证数据展示

  • FC experiment of human PBMCs using 65530-1-PBS

    FC experiment of human PBMCs using 65530-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65530-1-PBS

    FC experiment of human PBMCs using 65530-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65530-1-PBS targets CD5 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human T-cell acute lymphoblastic leukemia (T-ALL) cells 种属同源性预测
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Full Name CD5 molecule
Synonyms CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1
Calculated Molecular Weight 495 aa, 55 kDa
GenBank Accession NumberBC027901
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
FC protocol for CD5 antibody 65530-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols