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Anti-Human CD5 Rabbit Recombinant Antibody

CD5 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240428A12

Cat no : 98054-1-RR

Print datasheet

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

验证数据展示

  • FC experiment of human PBMCs using 98054-1-RR

    FC experiment of human PBMCs using 98054-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Rabbit Recombinant Antibody (98054-1-RR, Clone: 240428A12) or 0.25 ug Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98054-1-RR

    FC experiment of human PBMCs using 98054-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Rabbit Recombinant Antibody (98054-1-RR, Clone: 240428A12) (red) or 0.25 ug Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98054-1-RR

    Affinity and Kinetic Characterization of 98054-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98054-1-RR against Human CD5 were performed. The affinity constant is 0.27 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98054-1-RR is CD5 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD5 molecule
Synonyms T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule
Calculated Molecular Weight55 kDa
GenBank Accession NumberNM_014207.4
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06127
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
FC protocol for CD5 antibody 98054-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols