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Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a Recombinant Antibody, PBS Only

ICAM-1/CD54 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

15.2

Cat no : 65567-1-PBS

Print datasheet

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

验证数据展示

  • FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

    FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD54 Mouse IgG2a Recombinant Antibody (65567-1-MR, Clone:15.2) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65567-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

    FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD54 Mouse IgG2a Recombinant Antibody (65567-1-MR, Clone:15.2) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65567-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

产品信息

65567-1-PBS targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54, ICAM-1, ICAM1, ICAM 1, BB2
Calculated Molecular Weight 90 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).