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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using PE-65067

    FC experiment of human peripheral blood lymphocytes using PE-65067

    1X10^6 human peripheral blood lymphocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Human CD3 (CL647-65151, Clone:UCHT1) and 0.2 ug PE Anti-Human CD56 (PE-65067, Clone: MEM 188). Cells were not fixed.

  • FC experiment of human peripheral blood lymphocytes using PE-65067

    FC experiment of human peripheral blood lymphocytes using PE-65067

    1X10^6 human peripheral blood lymphocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Human CD3 (CL647-65151, Clone:UCHT1) and 0.2 ug PE-Mouse IgG2a Isotype Control. Cells were not fixed.

PE Anti-Human NCAM1/CD56 (MEM-188)

NCAM1/CD56 Monoclonal Antibody for FC
Cat No. PE-65067

Print datasheet

CloneNo. MEM-188

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

FC

Synonyms

CD56, NCAM1, NCAM 1, NCAM, N CAM 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy NCAM1/CD56 Ready-To-Use IHC Kit. NCAM1/CD56 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 ul per 10^6 cells in a 100 µl suspension or 10 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65067 targets NCAM1/CD56 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Acute myelogenous leukemia cell line KG-1 种属同源性预测
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name neural cell adhesion molecule 1
Synonyms CD56, NCAM1, NCAM 1, NCAM, N CAM 1
GenBank Accession NumberBC014205
Gene Symbol NCAM1
Gene ID (NCBI) 4684
ENSEMBL Gene IDENSG00000149294
RRIDAB_2883864
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP13591
Storage Buffer PBS with 0.1% sodium azide and a stabilizer.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas.

实验方案

Product Specific Protocols
FC protocol for PE NCAM1/CD56 antibody PE-65067Download protocol
Standard Protocols
Click here to view our Standard Protocols