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CD58 Polyclonal antibody

CD58 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 10878-1-AP

Print datasheet

Synonyms

Ag3, CD58, CD58 molecule, LFA 3, LFA3, Surface glycoprotein LFA 3

验证数据展示

  • WB analysis using 10878-1-AP

    WB analysis using 10878-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells, Jurkat cells, TF-1 cells, HeLa cells, human lymph tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10878-1-AP targets CD58 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CD58 fusion protein Ag1165 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD58 molecule
Synonyms Ag3, CD58, CD58 molecule, LFA 3, LFA3, Surface glycoprotein LFA 3
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC005930
Gene Symbol CD58
Gene ID (NCBI) 965
RRIDAB_2260179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19256
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD58 a heavily glycosylated protein of 55-70 kDa, is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells. The protein is localized to the plasma membrane.

实验方案

Product Specific Protocols
WB protocol for CD58 antibody 10878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols