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验证数据展示

  • WB analysis using 10878-1-AP

    WB analysis using 10878-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 10878-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 10878-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10878-1-AP (CD58 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 10878-1-AP

    IHC staining of human ovary cancer using 10878-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10878-1-AP (CD58 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 10878-1-AP

    IHC staining of human skin cancer using 10878-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10878-1-AP (CD58 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD58 Polyclonal antibody

CD58 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10878-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, Jurkat cells, TF-1 cells, HeLa cells, human lymph tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10878-1-AP targets CD58 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 CD58 fusion protein Ag1165 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD58 molecule
别名 Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule
计算分子量 28 kDa
观测分子量 55-70 kDa
GenBank蛋白编号BC005930
基因名称 CD58
Gene ID (NCBI) 965
RRIDAB_2260179
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19256
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD58 a heavily glycosylated protein of 55-70 kDa, is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells. The protein is localized to the plasma membrane.

实验方案

Product Specific Protocols
WB protocol for CD58 antibody 10878-1-APDownload protocol
IHC protocol for CD58 antibody 10878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Heliyon

Clinical significance of immune-related antigen CD58 in gliomas and analysis of its potential core related gene clusters

Authors - Zhi Tian
View Article