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验证数据展示

  • WB analysis using 30223-1-AP

    WB analysis using 30223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30223-1-AP (CD59 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 30223-1-AP

    IHC staining of human placenta using 30223-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30223-1-AP (CD59 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD59 antibody included
  • IHC staining of human tonsillitis using 30223-1-AP

    IHC staining of human tonsillitis using 30223-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 30223-1-AP (CD59 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD59 antibody included
  • IHC staining of human placenta using 30223-1-AP

    IHC staining of human placenta using 30223-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30223-1-AP (CD59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD59 antibody included
  • IF Staining of BxPC-3 using 30223-1-AP

    IF Staining of BxPC-3 using 30223-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using CD59 antibody (30223-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CD59 Polyclonal antibody

CD59 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30223-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

MAC-inhibitory protein, HRF-20, CD59 glycoprotein, CD59 antigen, CD 59

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HT-1080 cells, HT-29 cells, HUVEC cells, HeLa cells, U-87 MG cells
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30223-1-AP targets CD59 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CD59 fusion protein Ag32557 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD59 molecule, complement regulatory protein
别名 MAC-inhibitory protein, HRF-20, CD59 glycoprotein, CD59 antigen, CD 59
计算分子量 19 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC001506
基因名称 CD59
Gene ID (NCBI) 966
RRIDAB_3086267
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13987
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

实验方案

Product Specific Protocols
WB protocol for CD59 antibody 30223-1-APDownload protocol
IHC protocol for CD59 antibody 30223-1-APDownload protocol
IF protocol for CD59 antibody 30223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols