CD59 Polyclonal antibody
CD59 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | BxPC-3 cells, HT-1080 cells, HT-29 cells, HUVEC cells, HeLa cells, U-87 MG cells |
Positive IHC detected in | human placenta tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | BxPC-3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30223-1-AP targets CD59 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | CD59 fusion protein Ag32557 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD59 molecule, complement regulatory protein |
Synonyms | MAC-inhibitory protein, HRF-20, CD59 glycoprotein, CD59 antigen, CD 59 |
Calculated Molecular Weight | 19 kDa |
Observed Molecular Weight | 18-20 kDa |
GenBank Accession Number | BC001506 |
Gene Symbol | CD59 |
Gene ID (NCBI) | 966 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P13987 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CD59 antibody 30223-1-AP | Download protocol |
IHC protocol for CD59 antibody 30223-1-AP | Download protocol |
IF protocol for CD59 antibody 30223-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |