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CoraLite® Plus 647 Anti-Mouse CD62L (L-Selectin) (MEL-14)

CD62L (L-Selectin) Monoclonal Antibody for IF, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

MEL-14

Cat no : CL647-65123

Print datasheet

Synonyms

CD62L (L-Selectin), Sell

验证数据展示

  • FC experiment of mouse splenocytes using CL647-65123

    FC experiment of mouse splenocytes using CL647-65123

    1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse CD62L (L-Selectin) (CL647-65123, Clone: MEL-14). Cells were not fixed.

  • FC experiment of mouse splenocytes using CL647-65123

    FC experiment of mouse splenocytes using CL647-65123

    1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and CoraLite® Plus 647-conjugated Rat IgG2a isotype control antibody. Cells were not fixed.

  • IF Staining of mouse splenocytes using CL647-65123

    IF Staining of mouse splenocytes using CL647-65123

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated CD62L (L-Selectin) antibody (CL647-65123, Clone: MEL-14, red), and CoraLite®488-conjugated CD3 antibody (CL488-65077, Clone: 17A2, green).

经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65123 targets CD62L (L-Selectin) in IF, FC applications and shows reactivity with Mouse samples.

Tested Applications IF, FC Application Description
Tested Reactivity Mouse
Immunogen C3H/eb mouse B lymphoma 38C-13 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name selectin, lymphocyte
Synonyms CD62L (L-Selectin), Sell
GenBank Accession NumberBC052681
Gene Symbol CD62L
Gene ID (NCBI) 20343
RRIDAB_2883696
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP18337
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CD62L (L-Selectin) antibody CL647-65123Download protocol
FC protocol for CL Plus 647 CD62L (L-Selectin) antibody CL647-65123Download protocol
Standard Protocols
Click here to view our Standard Protocols