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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human peripheral blood platelets using 65295-1-Ig

    FC experiment of human peripheral blood platelets using 65295-1-Ig

    100ul thrombin-activated (red) or unactivated (black dashed) human peripheral blood platelets (diluted 1:2 in PBS) were surface stained with 0.2 ug Anti-Human CD63 (65295-1-Ig, Clone: H5C6) or Mouse IgG1 Isotype Control (65124-1-Ig) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

  • FC experiment of human peripheral blood platelets using 65295-1-Ig

    FC experiment of human peripheral blood platelets using 65295-1-Ig

    100ul thrombin-activated (red) or unactivated (black dashed) human peripheral blood platelets (diluted 1:2 in PBS) were surface stained with 0.2 ug Anti-Human CD63 (65295-1-Ig, Clone: H5C6) or Mouse IgG1 Isotype Control (65124-1-Ig) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

Anti-Human CD63 (H5C6)

CD63 Monoclonal Antibody for FC
Cat No. 65295-1-Ig

Print datasheet

CloneNo. H5C6

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, H5C6, CD63 antigen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC detected inthrombin-activated human peripheral blood platelets
Planning an IHC experiment? We recommend our IHCeasy CD63 Ready-To-Use IHC Kit. CD63 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65295-1-Ig targets CD63 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human splenic adherent cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD63 molecule
Synonyms Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, H5C6, CD63 antigen
GenBank Accession NumberBC013017
Gene Symbol CD63
Gene ID (NCBI) 967
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

实验方案

Product Specific Protocols
FC protocol for CD63 antibody 65295-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols