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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of human peripheral blood platelets using 65295-1-Ig

    FC experiment of human peripheral blood platelets using 65295-1-Ig

    100ul thrombin-activated (red) or unactivated (black dashed) human peripheral blood platelets (diluted 1:2 in PBS) were surface stained with 0.2 ug Anti-Human CD63 (65295-1-Ig, Clone: H5C6) or Mouse IgG1 Isotype Control (65124-1-Ig) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

  • FC experiment of human peripheral blood platelets using 65295-1-Ig

    FC experiment of human peripheral blood platelets using 65295-1-Ig

    100ul thrombin-activated (red) or unactivated (black dashed) human peripheral blood platelets (diluted 1:2 in PBS) were surface stained with 0.2 ug Anti-Human CD63 (65295-1-Ig, Clone: H5C6) or Mouse IgG1 Isotype Control (65124-1-Ig) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

Anti-Human CD63 (H5C6)

CD63 Monoclonal Antibody for FC
Cat No. 65295-1-Ig

产品说明书

CloneNo. H5C6

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC

别名

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, H5C6, CD63 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inthrombin-activated human peripheral blood platelets
Planning an IHC experiment? We recommend our IHCeasy CD63 Ready-To-Use IHC Kit. CD63 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65295-1-Ig targets CD63 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human splenic adherent cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD63 molecule
别名 Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, H5C6, CD63 antigen
GenBank蛋白编号BC013017
基因名称 CD63
Gene ID (NCBI) 967
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

实验方案

Product Specific Protocols
FC protocol for CD63 antibody 65295-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols