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Anti-Human CD68 (KP1)

CD68 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

KP1

Cat no : 65202-1-Ig

Print datasheet

Synonyms

CD68, CD68 molecule, GP110, KP1, Macrosialin, SCARD1

验证数据展示

  • FC experiment of human PBMCs using 65202-1-Ig

    FC experiment of human PBMCs using 65202-1-Ig

    1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65202-1-Ig, Clone:KP1) and FITC-Donkey anti-Mouse IgG at dilution 1:1000 (red), or Mouse IgG1 Isotype Control and FITC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using 65202-1-Ig

    FC experiment of human PBMCs using 65202-1-Ig

    1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65202-1-Ig, Clone:KP1) and FITC-Donkey anti-Mouse IgG at dilution 1:1000, or Mouse IgG1 Isotype Control and FITC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65202-1-Ig targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen Lysosomal fraction of human alveolar macrophages 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD68 molecule
Synonyms CD68, CD68 molecule, GP110, KP1, Macrosialin, SCARD1
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP34810
Storage Buffer PBS with 0.1% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
FC protocol for CD68 antibody 65202-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols