• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD68 Recombinant antibody

CD68 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241810B3

Cat no : 84596-4-RR

Print datasheet

Synonyms

SCARD1, Macrosialin, GP110, CD68 molecule, 241810B3

验证数据展示

  • WB analysis using 84596-4-RR

    WB analysis using 84596-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84596-4-RR (CD68 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84596-4-RR

    IHC staining of human tonsillitis using 84596-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included
  • IHC staining of human tonsillitis using 84596-4-RR

    IHC staining of human tonsillitis using 84596-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included

经过测试的应用

Positive WB detected inJ774A.1 cells, U-937 cells, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84596-4-RR targets CD68 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD68 molecule
Synonyms SCARD1, Macrosialin, GP110, CD68 molecule, 241810B3
Calculated Molecular Weight 37 kDa
Observed Molecular Weight80-90 kDa
GenBank Accession NumberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP34810
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in the phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
WB protocol for CD68 antibody 84596-4-RRDownload protocol
IHC protocol for CD68 antibody 84596-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols