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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using FITC-65515

    FC experiment of human PBMCs using FITC-65515

    1x10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 Rabbit Recombinant Antibody (FITC-65515, Clone:KP1) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

  • FC experiment of human PBMCs using FITC-65515

    FC experiment of human PBMCs using FITC-65515

    1x10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 Rabbit Recombinant Antibody (FITC-65515, Clone:KP1) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

FITC Plus Anti-Human CD68 (KP1) Rabbit IgG Recombinant Antibody

CD68 Recombinant Antibody for FC (Intra)
Cat No. FITC-65515

产品说明书

CloneNo. KP1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

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经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65515 targets CD68 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Lysosomal fraction of human alveolar macrophages 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD68 molecule
别名 SCARD1, Macrosialin, KP1, GP110, CD68 molecule
计算分子量 37 kDa
GenBank蛋白编号BC015557
基因名称 CD68
Gene ID (NCBI) 968
RRIDAB_3673856
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP34810
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD68 antibody FITC-65515Download protocol
Standard Protocols
Click here to view our Standard Protocols