CD69 Polyclonal antibody

CD69 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10803-1-AP

Print datasheet

Synonyms

CLEC2C, CD69 molecule, BL-AC/P26, BL AC/P26, AIM



经过测试的应用

Positive WB detected inhuman peripheral blood platelets
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10803-1-AP targets CD69 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen CD69 fusion protein Ag1094 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD69 molecule
Synonyms CLEC2C, CD69 molecule, BL-AC/P26, BL AC/P26, AIM
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 28 kDa, 32 kDa
GenBank Accession NumberBC007037
Gene Symbol CD69
Gene ID (NCBI) 969
RRIDAB_2074965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07108
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD69, also known as activation inducer molecule (AIM) or early activation antigen (EA1), is a cell surface glycoprotein expressed shortly after activation on T lymphocytes and other cells of haematopoietic origin, including natural killer cells. It is a disulfide-linked homodimer composed of two chains belonging to the type II integral protein with a C-type lectin domain. CD69 is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, natural killer cells, and platelets.

实验方案

Product Specific Protocols
WB protocol for CD69 antibody 10803-1-APDownload protocol
IHC protocol for CD69 antibody 10803-1-APDownload protocol
IF protocol for CD69 antibody 10803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Cancer Commun (Lond)

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies

Authors - Lu Tang
humanIF

iScience

Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis

Authors - Mick J M van Eijs
humanIF

Expert Rev Clin Immunol

Juxtaposition of IL-1β and IFN-γ expression and apoptosis of keratinocytes in adult-onset Still's disease.

Authors - Shijia Rao
IF

Cell Rep

Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.

Authors - Huiwen Zheng
humanIHC

Oncoimmunology

Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.

Authors - Chen Zhu
mouseFC

J Nanobiotechnology

Optogenetic engineered umbilical cord MSC-derived exosomes for remodeling of the immune microenvironment in diabetic wounds and the promotion of tissue repair

Authors - Xin Zhao