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CD69 Monoclonal antibody

CD69 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D10A2

Cat no : 68827-1-Ig

Print datasheet

Synonyms

Activation inducer molecule, AIM, BL AC/P26, CD69, CD69 molecule, CLEC2C, EA1, Early activation antigen CD69, GP32/28, MLR 3

验证数据展示

  • WB analysis using 68827-1-Ig

    WB analysis using 68827-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68827-1-Ig (CD69 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inU-937 cells, THP-1 cells, HL-60 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68827-1-Ig targets CD69 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen CD69 fusion protein Ag30963 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name CD69 molecule
Synonyms Activation inducer molecule, AIM, BL AC/P26, CD69, CD69 molecule, CLEC2C, EA1, Early activation antigen CD69, GP32/28, MLR 3
Calculated Molecular Weight 23 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC007037
Gene Symbol CD69
Gene ID (NCBI) 969
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07108
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, and several subsets of tissue-resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in regulating immune responses (PMID: 15745855).

实验方案

Product Specific Protocols
WB protocol for CD69 antibody 68827-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols