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Species Reactivity
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验证数据展示

  • FC experiment of human PBMCs using 98173-1-RR (same clone as 98173-1-PBS)

    FC experiment of human PBMCs using 98173-1-RR (same clone as 98173-1-PBS)

    1x10^6 untreated or PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-1-RR, Clone:241552F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD4 (RPA-T4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98173-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98173-1-RR (same clone as 98173-1-PBS)

    FC experiment of human PBMCs using 98173-1-RR (same clone as 98173-1-PBS)

    1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-1-RR, Clone:241552F1) (red) or 0.25 ug Isotype Control, or 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-1-RR, Clone:241552F1)(black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98173-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98173-1-RR

    Affinity and Kinetic Characterization of 98173-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98173-1-RR against Human CD69 were performed. The affinity constant is 0.257 nM.

Anti-Human CD69 Rabbit Recombinant Antibody, PBS Only

CD69 Recombinant Antibody for FC
Cat No. 98173-1-PBS

产品说明书

CloneNo. 241552F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98173-1-PBS targets CD69 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD69 molecule
别名 CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule
计算分子量23 kDa
GenBank蛋白编号NM_001781.2
基因名称 CD69
Gene ID (NCBI) 969
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ07108
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).