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验证数据展示

  • IF Staining of THP-1 using 84455-2-RR

    IF Staining of THP-1 using 84455-2-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD70 antibody (84455-2-RR, Clone: 241324B12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84455-2-RR

    Affinity and Kinetic Characterization of 84455-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84455-2-RR against Human CD70 were performed. The affinity constant is 67.0 pM.

CD70 Recombinant antibody

CD70 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84455-2-RR

Print datasheet

CloneNo. 241324B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

CD27LG, CD27-L, CD27L, CD27 ligand, CD27 L

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84455-2-RR targets CD70 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD70 molecule
Synonyms CD27LG, CD27-L, CD27L, CD27 ligand, CD27 L
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC000725
Gene Symbol CD70
Gene ID (NCBI) 970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32970
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CD70 antibody 84455-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols