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CD71 Monoclonal antibody

CD71 Monoclonal Antibody for WB, IHC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, mouse

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

H68.4

Cat no : 65236-1-Ig

Print datasheet

Synonyms

TFR1, TFR, T9, sTfR, p90

验证数据展示

  • WB analysis using 65236-1-Ig

    WB analysis using 65236-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 65236-1-Ig (CD71 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 65236-1-Ig

    IHC staining of human placenta using 65236-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD71 antibody included
  • IHC staining of human placenta using 65236-1-Ig

    IHC staining of human placenta using 65236-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD71 antibody included

经过测试的应用

Positive WB detected inPC-3 cells, RAW 264.7 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65236-1-Ig targets CD71 in WB, IHC applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC Application Description
Tested Reactivity human, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name transferrin receptor (p90, CD71)
Synonyms TFR1, TFR, T9, sTfR, p90
Calculated Molecular Weight 85 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC001188
Gene Symbol CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP02786
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

实验方案

Product Specific Protocols
WB protocol for CD71 antibody 65236-1-IgDownload protocol
IHC protocol for CD71 antibody 65236-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols