验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, RAW 264.7 cells, HeLa cells, K-562 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
65236-1-Ig targets CD71 in WB, IHC applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse, rat |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transferrin receptor (p90, CD71) |
| 别名 | TFR1, TFR, T9, sTfR, p90 |
| 计算分子量 | 85 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC001188 |
| 基因名称 | CD71 |
| Gene ID (NCBI) | 7037 |
| ENSEMBL Gene ID | ENSG00000072274 |
| RRID | AB_3085030 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P02786 |
| 储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD71 antibody 65236-1-Ig | Download protocol |
| IHC protocol for CD71 antibody 65236-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Med TIMP1 regulates ferroptosis in osteoblasts by inhibiting TFRC ubiquitination: an in vitro and in vivo study | ||
J Ethnopharmacol The extract from Quzhou Aurantii Fructus attenuates cough variant asthma through inhibiting the TRPV1/Ca2+/NFAT/TSLP pathway and ferroptosis via TRPV1 mediation in ovalbumin-induced mice | ||
Hum Exp Toxicol KLF6 silencing attenuates MCAO-induced brain injury and cognitive dysfunction via targeting ferroptosis and activating the Nrf2/HO-1 pathway | ||
Int Immunopharmacol Urolithin A improves myocardial ischemia-reperfusion injury by attenuating oxidative stress and ferroptosis through Nrf2 pathway |
