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CoraLite® Plus 647 Anti-Human CD71 (OKT9) Rabbit Recombinant Antibody

CD71 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OKT9

Cat No : CL647-65507

Print datasheet

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

验证数据展示

  • FC experiment of human PBMCs using CL647-65507

    FC experiment of human PBMCs using CL647-65507

    1X10^6 PHA-activated (3 day) human PBMCs were surface stained with FITC Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD71 Rabbit Recombinant Antibody (CL647-65507, Clone:OKT9) (right) or CoraLite® Plus 647-conjugated Rabbit IgG control (CL647-30000) (left). 1X10^6 unactivated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD71 Mouse Monoclonal Antibody (center). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL647-65507

    FC experiment of human PBMCs using CL647-65507

    1X10^6 PHA-activated (3 day) human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD71 Rabbit Recombinant Antibody (CL647-65507, Clone:OKT9) (red) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000) (blue). 1X10^6 unactivated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD71 Mouse Monoclonal Antibody (black dashed). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inPHA treated (3 day) human PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65507-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65507 targets CD71 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human T-cell acute lymphoblastic leukemia cells 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transferrin receptor (p90, CD71)
Synonyms CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR
Calculated Molecular Weight 85 kDa
GenBank Accession NumberBC001188
Gene Symbol TFRC
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD71 antibody CL647-65507Download protocol
Standard Protocols
Click here to view our Standard Protocols