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验证数据展示

  • WB analysis of Raji using 84270-5-RR

    WB analysis of Raji using 84270-5-RR

    Raji cells were subjected to SDS PAGE followed by western blot with 84270-5-RR (CD72 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84270-5-RR

    Affinity and Kinetic Characterization of 84270-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84270-5-RR against Human CD72 were performed. The affinity constant is 59.3 pM.

CD72 Recombinant antibody

CD72 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84270-5-RR

产品说明书

CloneNo. 241301B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

LYB2, Lyb 2, CD72b, CD72 molecule, B-cell differentiation antigen CD72

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84270-5-RR targets CD72 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD72 molecule
别名 LYB2, Lyb 2, CD72b, CD72 molecule, B-cell differentiation antigen CD72
计算分子量 359 aa, 40 kDa
GenBank蛋白编号BC030227
基因名称 CD72
Gene ID (NCBI) 971
RRIDAB_3671816
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP21854
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

实验方案

Product Specific Protocols
WB protocol for CD72 antibody 84270-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols