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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using PE-65157

    FC experiment of human peripheral blood lymphocytes using PE-65157

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD19 (APC-65110, Clone:HIB19) and 20.00 ul/test PE Anti-Human CD72 (PE-65157, Clone:3F3). Cells were not fixed.

  • FC experiment of human peripheral blood lymphocytes using PE-65157

    FC experiment of human peripheral blood lymphocytes using PE-65157

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD19 (APC-65110, Clone:HIB19) and PE mouse IgG2b isotype control antibody. Cells were not fixed.

PE Anti-Human CD72 (3F3)

CD72 Monoclonal Antibody for FC
Cat No. PE-65157

产品说明书

CloneNo. 3F3

宿主/亚型

Mouse / IgG2b, kappa

种属反应性

Human

应用

FC

别名

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

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经过测试的应用

Positive FC detected inHuman peripheral blood lymphocytes

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65157 targets CD72 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 Normal human lymphocytes from a lymph node 种属同源性预测
宿主/亚型 Mouse / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD72 molecule
别名 CD72, CD72 molecule, CD72b, Lyb 2, LYB2
GenBank蛋白编号BC011734
基因名称 CD72
Gene ID (NCBI) 971
RRIDAB_2883935
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDP21854
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

实验方案

Product Specific Protocols
FC protocol for PE CD72 antibody PE-65157Download protocol
Standard Protocols
Click here to view our Standard Protocols