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Anti-Human CD73 (AD2) Rabbit Recombinant Antibody

CD73 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

AD2

Cat no : 65500-1-RR

Print datasheet

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE

验证数据展示

  • FC experiment of human PBMCs using 65500-1-RR

    FC experiment of human PBMCs using 65500-1-RR

    1X10^6 human PBMCs were surface stained with CL488 Anti-Human CD3 and 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) or Rabbit IgG control PolyAb. Unconjugated antibodies were labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) prior to staining. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65500-1-RR

    FC experiment of human PBMCs using 65500-1-RR

    1X10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) or Rabbit IgG control PolyAb. Unconjugated antibodies were labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) prior to staining. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65500-1-RR targets CD73 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Pre-B leukemia cell line 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 5'-nucleotidase, ecto (CD73)
Synonyms 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE
Calculated Molecular Weight 29 kDa, 63 kDa
GenBank Accession NumberBC015940
Gene Symbol CD73
Gene ID (NCBI) 4907
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

实验方案

Product Specific Protocols
FC protocol for CD73 antibody 65500-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols