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Species Reactivity
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验证数据展示

  • FC experiment of human PBMCs using 65531-1-PBS

    FC experiment of human PBMCs using 65531-1-PBS

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD73 (65531-1-PBS, Clone: AD2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.25 ug Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (blue). Cells were not fixed.

  • FC experiment of human PBMCs using 65531-1-MR (same clone as 65531-1-PBS)

    FC experiment of human PBMCs using 65531-1-MR (same clone as 65531-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD73 (AD2) Mouse IgG1 RecAb (65531-1-MR, Clone:AD2) or Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD45. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65531-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65531-1-MR (same clone as 65531-1-PBS)

    FC experiment of human PBMCs using 65531-1-MR (same clone as 65531-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD73 (AD2) Mouse IgG1 RecAb (65531-1-MR, Clone:AD2) (red) or Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65531-1-PBS in a different storage buffer formulation.

Anti-Human CD73 (AD2) Mouse IgG1 Recombinant Antibody, PBS Only

CD73 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65531-1-PBS

产品说明书

CloneNo. AD2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC

别名

NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65531-1-PBS targets CD73 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Recombinant
产品类型 Antibody
全称 5'-nucleotidase, ecto (CD73)
别名 NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase
计算分子量 29 kDa, 63 kDa
GenBank蛋白编号BC015940
基因名称 CD73
Gene ID (NCBI) 4907
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

实验方案

Product Specific Protocols
FC protocol for CD73 antibody 65531-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols