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FITC Plus Anti-Human CD73 (AD2) Rabbit Recombinant Antibody

CD73 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

AD2

Cat no : FITC-65500

Print datasheet

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73)

验证数据展示

  • FC experiment of human PBMCs using FITC-65500

    FC experiment of human PBMCs using FITC-65500

    1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul FITC Plus Anti-Human CD73 Rabbit Recombinant Antibody (FITC-65500, Clone:AD2) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using FITC-65500

    FC experiment of human PBMCs using FITC-65500

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD73 Rabbit Recombinant Antibody (FITC-65500, Clone:AD2) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65500 targets CD73 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Pre-B leukemia cell line 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 5'-nucleotidase, ecto (CD73)
Synonyms 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73)
Calculated Molecular Weight 29 kDa, 63 kDa
GenBank Accession NumberBC015940
Gene Symbol CD73
Gene ID (NCBI) 4907
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD73 antibody FITC-65500Download protocol
Standard Protocols
Click here to view our Standard Protocols