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CD79a Recombinant antibody

CD79a Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F24

Cat no : 82747-4-RR

Print datasheet

Synonyms

MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain

验证数据展示

  • WB analysis using 82747-4-RR

    WB analysis using 82747-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82747-4-RR (CD79a antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82747-4-RR

    Affinity and Kinetic Characterization of 82747-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82747-4-RR against Human CD79A were performed. The affinity constant is 4.12 nM.

经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Planning an IHC experiment? We recommend our IHCeasy CD79a Ready-To-Use IHC Kit. CD79a primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82747-4-RR targets CD79a in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CD79a fusion protein Ag17924 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD79a molecule, immunoglobulin-associated alpha
Synonyms MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain
Calculated Molecular Weight 226 aa, 25 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC113733
Gene Symbol CD79A
Gene ID (NCBI) 973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11912
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

实验方案

Product Specific Protocols
WB protocol for CD79a antibody 82747-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols