• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD79b Recombinant antibody

CD79b Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241273B7

Cat no : 84234-5-RR

Print datasheet

Synonyms

IGB, Ig beta, B-cell-specific glycoprotein B29, B-cell antigen receptor complex-associated protein beta chain, B29

验证数据展示

  • WB analysis using 84234-5-RR

    WB analysis using 84234-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84234-5-RR (CD79b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 84234-5-RR

    IF Staining of Raji using 84234-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD79b antibody (84234-5-RR, Clone: 241273B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84234-5-RR

    Affinity and Kinetic Characterization of 84234-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84234-5-RR against Human CD79b were performed. The affinity constant is 3.23 nM.

经过测试的应用

Positive WB detected inRaji cells, Ramos cells, Daudi cells
Positive IF/ICC detected inRaji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84234-5-RR targets CD79b in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD79b molecule, immunoglobulin-associated beta
Synonyms IGB, Ig beta, B-cell-specific glycoprotein B29, B-cell antigen receptor complex-associated protein beta chain, B29
Calculated Molecular Weight 229 aa, 26 kDa
Observed Molecular Weight32-36 kDa
GenBank Accession NumberBC032651
Gene Symbol CD79B
Gene ID (NCBI) 974
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP40259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).

实验方案

Product Specific Protocols
WB protocol for CD79b antibody 84234-5-RRDownload protocol
IF protocol for CD79b antibody 84234-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols