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Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody, PBS Only

CD8 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

UCHT4

Cat no : 65559-1-PBS

Print datasheet

Synonyms

CD8A, T-cell surface glycoprotein CD8 alpha chain, p32, MAL, Leu2

验证数据展示

  • FC experiment of human PBMCs using 65559-1-MR (same clone as 65559-1-PBS)

    FC experiment of human PBMCs using 65559-1-MR (same clone as 65559-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (65559-1-MR, Clone: UCHT4) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65559-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65559-1-MR (same clone as 65559-1-PBS)

    FC experiment of human PBMCs using 65559-1-MR (same clone as 65559-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (65559-1-MR, Clone: UCHT4) (red) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65559-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.

产品信息

65559-1-PBS targets CD8 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Thymocytes and Sézary T cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD8a molecule
Synonyms CD8A, T-cell surface glycoprotein CD8 alpha chain, p32, MAL, Leu2
Calculated Molecular Weight 235 aa, 26 kDa
GenBank Accession NumberBC025715
Gene Symbol CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01732
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).