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验证数据展示

  • WB analysis using 84644-1-RR

    WB analysis using 84644-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84644-1-RR (CD81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HCT 116 using 84644-1-RR

    WB analysis of HCT 116 using 84644-1-RR

    WB result of CD81 antibody (84644-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and CD81 knockout HCT 116 cells.

  • Affinity and Kinetic Characterization of 84644-1-RR

    Affinity and Kinetic Characterization of 84644-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84644-1-RR against Human CD81 were performed. The affinity constant is 0.602 nM.

  • Featured Product
  • KD/KO Validated

CD81 Recombinant antibody

CD81 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84644-1-RR

Print datasheet

CloneNo. 241722B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TAPA1, S5.7, CD81 molecule, CD81 antigen, 26 kDa cell surface protein TAPA-1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, HCT 116 cells, Ramos cells, Daudi cells, Jurkat cells, THP-1 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84644-1-RR targets CD81 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD81 molecule
Synonyms TAPA1, S5.7, CD81 molecule, CD81 antigen, 26 kDa cell surface protein TAPA-1
Calculated Molecular Weight26 kDa
Observed Molecular Weight20-25 kDa
GenBank Accession NumberNM_004356.4
Gene Symbol CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
RRIDAB_3672103
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP60033
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

实验方案

Product Specific Protocols
WB protocol for CD81 antibody 84644-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols