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CD81 Recombinant antibody

CD81 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241722G4

Cat no : 84644-2-RR

Print datasheet

Synonyms

TAPA1, S5.7, CD81 molecule, CD81 antigen, 26 kDa cell surface protein TAPA-1

验证数据展示

  • IF Staining of Jurkat using 84644-2-RR

    IF Staining of Jurkat using 84644-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD81 antibody (84644-2-RR, Clone: 241722G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84644-2-RR

    Affinity and Kinetic Characterization of 84644-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84644-2-RR against Human CD81 were performed. The affinity constant is 0.129 nM.

经过测试的应用

Positive IF/ICC detected inJurkat cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84644-2-RR is CD81 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD81 molecule
Synonyms TAPA1, S5.7, CD81 molecule, CD81 antigen, 26 kDa cell surface protein TAPA-1
Calculated Molecular Weight26kDa
GenBank Accession NumberNM_004356.4
Gene Symbol CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP60033
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

实验方案

Product Specific Protocols
IF protocol for CD81 antibody 84644-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols