• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody, PBS Only

CD83 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human, non-human primates

Applications

FC

Conjugate

Unconjugated

CloneNo.

HB15e

Cat no : 65581-1-PBS

Print datasheet

Synonyms

Cell surface protein HB15, CD83 molecule, CD83 antigen, BL11, B-cell activation protein

验证数据展示

  • FC experiment of human monocyte-derived mature dendritic cells using 65581-1-MR (same clone as 65581-1-PBS)

    FC experiment of human monocyte-derived mature dendritic cells using 65581-1-MR (same clone as 65581-1-PBS)

    1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human CD83 Mouse IgG2a Recombinant Antibody (65581-1-MR, Clone: HB15e) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65581-1-PBS in a different storage buffer formulation.

产品信息

65581-1-PBS targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

Tested Applications FC Application Description
Tested Reactivity human, non-human primates
Immunogen Human CD83-transfected Cos cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD83 molecule
Synonyms Cell surface protein HB15, CD83 molecule, CD83 antigen, BL11, B-cell activation protein
Calculated Molecular Weight 205 aa, 23 kDa
GenBank Accession NumberBC030830
Gene Symbol CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).