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CD83 Recombinant antibody, PBS Only (Detector)

CD83 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241855G5

Cat no : 84796-3-PBS

Print datasheet

Synonyms

CD83 molecule, CD83 antigen, BL11, B-cell activation protein, B cell activation protein

验证数据展示

  • WB analysis using 84796-3-RR (same clone as 84796-3-PBS)

    WB analysis using 84796-3-RR (same clone as 84796-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84796-3-RR (CD83 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84796-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01570-3

    Sandwich ELISA standard curve of MP01570-3

    Sandwich ELISA standard curve of MP01570-3, Human CD83 Recombinant Matched Antibody Pair - PBS only. 84796-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2033. 84796-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • Sample test of MP01570-3

    Sample test of MP01570-3

    Serum of six individual healthy human donors was measured. The human CD83 concentration of detected samples was determined to be 372.6 pg/mL with a range of 167.8 - 934.1 pg/mL.

  • Cytometric bead array standard curve of MP01570-1

    Cytometric bead array standard curve of MP01570-1

    Cytometric bead array standard curve of MP01570-1, CD83 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84796-1-PBS. Detection antibody: 84796-3-PBS. Standard: Eg2033. Range: 0.313-40 ng/mL.

产品信息

84796-3-PBS targets CD83 as part of a matched antibody pair:

MP01570-1: 84796-1-PBS capture and 84796-3-PBS detection (validated in Cytometric bead array)

MP01570-3: 84796-4-PBS capture and 84796-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD83 molecule
Synonyms CD83 molecule, CD83 antigen, BL11, B-cell activation protein, B cell activation protein
Calculated Molecular Weight23 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberNM_004233.4
Gene Symbol CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01151
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).