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Anti-Human CD86 (IT2.2)

CD86 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

IT2.2

Cat no : 65118-1-Ig

Print datasheet

Synonyms

Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

验证数据展示

  • FC experiment of human PBMCs using 65118-1-Ig

    FC experiment of human PBMCs using 65118-1-Ig

    1X10^6 human PBMCs were surface co-stained with FITC Anti-human CD14 and 0.2 ug Anti-Human CD86 (65118-1-Ig, Clone:IT2.2) and APC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using 65118-1-Ig

    FC experiment of human PBMCs using 65118-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD86 (65118-1-Ig, Clone:IT2.2) and APC-Goat anti-Mouse IgG at dilution 1:1000 (red) or unstained. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65118-1-Ig targets CD86 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD86 molecule
Synonyms Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72
Calculated Molecular Weight 329 aa, 38 kDa
GenBank Accession NumberBC040261
Gene Symbol CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42081
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

实验方案

Product Specific Protocols
FC protocol for CD86 antibody 65118-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols