验证数据展示
经过测试的应用
| Positive FC detected in | LPS-stimulated Wistar rat splenocytes |
Planning an IF experiment? We recommend our CoraLite®594 and CoraLite® Plus 750 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
| FC | See 1 publications below |
产品信息
65224-1-Ig targets CD86 in IF, FC applications and shows reactivity with rat samples.
| 经测试应用 | FC Application Description |
| 文献引用应用 | IF, FC |
| 经测试反应性 | rat |
| 文献引用反应性 | rat |
| 免疫原 | HTLV-1-transformed Lewis-S1 rat T cell line 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD86 molecule |
| 别名 | CD86 molecule, B7-2, B7 2, 24F |
| 计算分子量 | 36 kDa |
| GenBank蛋白编号 | NM_020081 |
| 基因名称 | Cd86 |
| Gene ID (NCBI) | 56822 |
| RRID | AB_3670278 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | O35531 |
| 储存缓冲液 | PBS with 0.09% sodium azide. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CD86 antibody 65224-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |