CD86 Recombinant antibody
CD86 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
230476A1
验证数据展示
经过测试的应用
| Positive WB detected in | DC2.4 cells, J774A.1 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 83213-5-RR is CD86 Fusion Protein expressed in E. coli.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | mouse |
| Immunogen | Fusion Protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | CD86 antigen |
| Synonyms | B7-2, B70, B7.2, B7 2, B7 |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | NM_019388 |
| Gene Symbol | Cd86 |
| Gene ID (NCBI) | 12524 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P42082-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD86 antibody 83213-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |