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CD86 Recombinant antibody

CD86 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240429G12

Cat no : 83523-4-RR

Print datasheet

Synonyms

BU63, B70, B7 2, Activation B7-2 antigen, Activation B7 2 antigen

验证数据展示

  • WB analysis using 83523-4-RR

    WB analysis using 83523-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83523-4-RR (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83523-4-RR

    IHC staining of human ovary cancer using 83523-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83523-4-RR (CD86 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 83523-4-RR

    IHC staining of human ovary cancer using 83523-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83523-4-RR (CD86 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83523-4-RR

    Affinity and Kinetic Characterization of 83523-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83523-4-RR against Human CD86 were performed. The affinity constant is 0.766 nM.

经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83523-4-RR is CD86 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD86 molecule
Synonyms BU63, B70, B7 2, Activation B7-2 antigen, Activation B7 2 antigen
Calculated Molecular Weight 329 aa, 38 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_175862
Gene Symbol CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP42081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

实验方案

Product Specific Protocols
WB protocol for CD86 antibody 83523-4-RRDownload protocol
IHC protocol for CD86 antibody 83523-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols