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FITC Plus Anti-Human CD86 (BU63) Rabbit Recombinant Antibody

CD86 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

BU63

Cat No : FITC-65514

Print datasheet

Synonyms

Activation B7 2 antigen, B7 2, B70

验证数据展示

  • FC experiment of human PBMCs using FITC-65514

    FC experiment of human PBMCs using FITC-65514

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • FC experiment of human PBMCs using FITC-65514

    FC experiment of human PBMCs using FITC-65514

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65514 targets CD86 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen B-lymphoblastoid cell line ARH 77 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD86 molecule
Synonyms Activation B7 2 antigen, B7 2, B70
Calculated Molecular Weight 329 aa, 38 kDa
GenBank Accession NumberBC040261
Gene Symbol CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD86 antibody FITC-65514Download protocol
Standard Protocols
Click here to view our Standard Protocols