CD8a Polyclonal antibody

CD8a Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29896-1-AP

Print datasheet

Synonyms

CD8, Ly B, Ly 35, Ly 2, CD8α



经过测试的应用

Positive WB detected inmouse spleen tissue, mouse thymus tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29896-1-AP targets CD8a in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity mouse
Cited Reactivityhuman, mouse
Immunogen CD8a fusion protein Ag31942 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD8 antigen, alpha chain
Synonyms CD8, Ly B, Ly 35, Ly 2, CD8α
Calculated Molecular Weight27KD
Observed Molecular Weight32-35 kDa
GenBank Accession NumberNM_001081110
Gene Symbol Cd8a
Gene ID (NCBI) 12525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01731
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 is composed of two disulfide-linked chains and can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

实验方案

Product Specific Protocols
WB protocol for CD8a antibody 29896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

Machine learning-based identification of tumor-infiltrating immune cell-associated model with appealing implications in improving prognosis and immunotherapy response in bladder cancer patients

Authors - Hualin Chen
mouse

Int Immunopharmacol

Triggering receptor expressed on myeloid cells-1 aggravates obliterative bronchiolitis via enhancing the proinflammatory phenotype of macrophages

Authors - Junhao Wan
mouse

Bioorg Chem

Synthesis and immunotherapy efficacy of a PD-L1 small-molecule inhibitor combined with its 131I-iodide labelled isostructural compound

Authors - Gaochao Lv
mouseIHC

Molecules

Immune Enhancement of Clam Peptides on Immunosuppressed Mice Induced by Hydrocortisone

Authors - Meibin Lv
mouseIHC

J Exp Clin Cancer Res

Regnase-1 downregulation promotes pancreatic cancer through myeloid-derived suppressor cell-mediated evasion of anticancer immunity

Authors - Junya Okabe
mouseIHC

Bioorg Chem

Discovery of selective and potent USP22 inhibitors via structure-based virtual screening and bioassays exerting anti-tumor activity

Authors - Yue Zhang