CD8a Polyclonal antibody
CD8a Polyclonal Antibody for WB, IF-P, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Mouse and More (2)
Applications
WB, IF-P, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse spleen tissue, mouse thymus tissue |
Positive IHC detected in | mouse thymus tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse spleen tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29896-1-AP targets CD8a in WB, IF, IHC, ELISA applications and shows reactivity with Mouse samples.
Tested Applications | WB, IF-P, IHC, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | Mouse |
Cited Reactivity | human, mouse |
Immunogen | CD8a fusion protein Ag31942 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD8 antigen, alpha chain |
Synonyms | CD8 antigen, alpha chain, Cd8a, Ly 2, Ly 35, Ly B, Lyt 2 |
Calculated Molecular Weight | 27KD |
Observed Molecular Weight | 32-35 kDa |
GenBank Accession Number | NM_001081110 |
Gene Symbol | Cd8a |
Gene ID (NCBI) | 12525 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P01731 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD8a antibody 29896-1-AP | Download protocol |
IHC protocol for CD8a antibody 29896-1-AP | Download protocol |
IF protocol for CD8a antibody 29896-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Immunol Machine learning-based identification of tumor-infiltrating immune cell-associated model with appealing implications in improving prognosis and immunotherapy response in bladder cancer patients | ||
Molecules Immune Enhancement of Clam Peptides on Immunosuppressed Mice Induced by Hydrocortisone | ||
J Exp Clin Cancer Res Regnase-1 downregulation promotes pancreatic cancer through myeloid-derived suppressor cell-mediated evasion of anticancer immunity | ||
Bioorg Chem Discovery of selective and potent USP22 inhibitors via structure-based virtual screening and bioassays exerting anti-tumor activity | ||
Biomed Pharmacother FGF19/FGFR4 signaling contributes to hepatocellular carcinoma survival and immune escape by regulating IGF2BP1-mediated expression of PD-L1 | ||
J Transl Med Potential crosstalk between SPP1 + TAMs and CD8 + exhausted T cells promotes an immunosuppressive environment in gastric metastatic cancer |