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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using 65604-1-MR (same clone as 65604-1-PBS)

    FC experiment of human PBMCs using 65604-1-MR (same clone as 65604-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8a (OKT8) Mouse IgG2a Recombinant Antibody (65604-1-MR, Clone:OKT8) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65604-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65604-1-MR (same clone as 65604-1-PBS)

    FC experiment of human PBMCs using 65604-1-MR (same clone as 65604-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8a (OKT8) Mouse IgG2a Recombinant Antibody (65604-1-MR, Clone: OKT8) (red) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65604-1-PBS in a different storage buffer formulation.

Anti-Human CD8a (OKT8) Mouse IgG2a Recombinant Antibody, PBS Only

CD8a Recombinant Antibody for FC
Cat No. 65604-1-PBS

产品说明书

CloneNo. OKT8

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

CD8, p32, OKT8, MAL, Leu2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.

产品信息

65604-1-PBS targets CD8a in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human thymocytes 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD8a molecule
别名 CD8, p32, OKT8, MAL, Leu2
计算分子量 235 aa, 26 kDa
GenBank蛋白编号BC025715
基因名称 CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T-cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).