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验证数据展示

  • WB analysis using 82105-1-RR

    WB analysis using 82105-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:24370 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 82105-1-RR

    IHC staining of human lung cancer using 82105-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human lung cancer using 82105-1-RR

    IHC staining of human lung cancer using 82105-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD9 antibody included
  • IF Staining of MCF-7 using 82105-1-RR

    IF Staining of MCF-7 using 82105-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (82105-1-RR, Clone: 5K14 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • WB analysis of human urine exosomes using 82105-1-RR

    WB analysis of human urine exosomes using 82105-1-RR

    human urine and human urine-derived exosomes were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82105-1-RR

    Affinity and Kinetic Characterization of 82105-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82105-1-RR against Human CD9 were performed. The affinity constant is 36.3 pM.

CD9 Recombinant antibody

CD9 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82105-1-RR

产品说明书

CloneNo. 5K14

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD9 antigen, BTCC 1, BA2, 5K14, 5H9 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human urine exosomes tissue, L02 cells, A431 cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:650-1:2600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82105-1-RR targets CD9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CD9 fusion protein Ag14546 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD9 molecule
别名 CD9 antigen, BTCC 1, BA2, 5K14, 5H9 antigen
计算分子量 228 aa, 25 kDa
观测分子量23-27 kDa
GenBank蛋白编号BC011988
基因名称 CD9
Gene ID (NCBI) 928
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21926
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).

实验方案

Product Specific Protocols
WB protocol for CD9 antibody 82105-1-RRDownload protocol
IHC protocol for CD9 antibody 82105-1-RRDownload protocol
IF protocol for CD9 antibody 82105-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols