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验证数据展示

  • WB analysis using 84142-2-RR

    WB analysis using 84142-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84142-2-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 84142-2-RR

    IF Staining of MCF-7 using 84142-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CD9 antibody (84142-2-RR, Clone: 240830A6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84142-2-RR

    Affinity and Kinetic Characterization of 84142-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84142-2-RR against Human CD9 were performed. The affinity constant is 1.57 nM.

CD9 Recombinant antibody

CD9 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84142-2-RR

产品说明书

CloneNo. 240830A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, L02 cells, human placenta tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84142-2-RR targets CD9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD9 molecule
别名 CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9
计算分子量25 kDa
观测分子量23 kDa
GenBank蛋白编号NM_001769.4
基因名称 CD9
Gene ID (NCBI) 928
RRIDAB_3671704
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP21926
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, which may be due to the difference in glycosylations (PMID: 8701996).

实验方案

Product Specific Protocols
WB protocol for CD9 antibody 84142-2-RRDownload protocol
IF protocol for CD9 antibody 84142-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols