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Biotin Anti-Human CD9 (MM2/57)

CD9 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC

Conjugate

Biotin

CloneNo.

MM2/57

Cat no : Biotin-65070

Print datasheet

Synonyms

CD9 molecule, CD9 antigen, BTCC 1, BA2, 5H9 antigen

验证数据展示

  • FC experiment of MCF-7 using Biotin-65070

    FC experiment of MCF-7 using Biotin-65070

    1X10^6 MCF-7 cells were surface stained with 5 μl Biotin Anti-Human CD9 (Biotin-65070, Clone: MM2/57) (red) or Control Antibody (blue), followed by Streptavidin PE. Cells were not fixed.

经过测试的应用

Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-65070 targets CD9 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD9 molecule
Synonyms CD9 molecule, CD9 antigen, BTCC 1, BA2, 5H9 antigen
Calculated Molecular Weight 228 aa, 25 kDa
GenBank Accession NumberBC011988
Gene Symbol CD9
Gene ID (NCBI) 928
Conjugate Biotin
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP21926
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).

实验方案

Product Specific Protocols
FC protocol for Biotin CD9 antibody Biotin-65070Download protocol
Standard Protocols
Click here to view our Standard Protocols