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验证数据展示

  • WB analysis using 84206-3-RR (same clone as 84206-3-PBS)

    WB analysis using 84206-3-RR (same clone as 84206-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84206-3-RR (CD90/Thy1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84206-3-PBS in a different storage buffer formulation.

  • IF Staining of PC-12 using 84206-3-RR (same clone as 84206-3-PBS)

    IF Staining of PC-12 using 84206-3-RR (same clone as 84206-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD90/Thy1 antibody (84206-3-RR, Clone: 241388E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84206-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01132-1

    Cytometric bead array standard curve of MP01132-1

    Cytometric bead array standard curve of MP01132-1, RAT CD90/Thy1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84206-3-PBS. Detection antibody: 84206-1-PBS. Standard: Eg1463. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP01132-2

    Cytometric bead array standard curve of MP01132-2

    Cytometric bead array standard curve of MP01132-2, RAT CD90/Thy1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84206-3-PBS. Detection antibody: 84206-2-PBS. Standard: Eg1463. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84206-3-RR

    Affinity and Kinetic Characterization of 84206-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84206-3-RR against Rat CD90/Thy1 were performed. The affinity constant is 27.1 pM.

Rat CD90/Thy1 Recombinant antibody, PBS Only (Capture)

CD90/Thy1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84206-3-PBS

产品说明书

CloneNo. 241388E2

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Thy-1 membrane glycoprotein, Thy-1 antigen, Thy-1, Thy1, CD90

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84206-3-PBS targets CD90/Thy1 as part of a matched antibody pair:

MP01132-1: 84206-3-PBS capture and 84206-1-PBS detection (validated in Cytometric bead array)

MP01132-2: 84206-3-PBS capture and 84206-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 rat
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Thy-1 cell surface antigen
别名 Thy-1 membrane glycoprotein, Thy-1 antigen, Thy-1, Thy1, CD90
计算分子量18 kDa
观测分子量25 kDa
GenBank蛋白编号NM_012673.2
基因名称 Thy1
Gene ID (NCBI) 24832
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01830
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.