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CD97 Polyclonal antibody, PBS Only

CD97 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13071-1-PBS

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Synonyms

CD97 molecule, CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5

验证数据展示

  • IHC staining of human tonsillitis using 13071-1-AP (same clone as 13071-1-PBS)

    IHC staining of human tonsillitis using 13071-1-AP (same clone as 13071-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13071-1-PBS in a different storage buffer formulation.

  • WB analysis using 13071-1-AP (same clone as 13071-1-PBS)

    WB analysis using 13071-1-AP (same clone as 13071-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13071-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 13071-1-AP (same clone as 13071-1-PBS)

    IHC staining of human tonsillitis using 13071-1-AP (same clone as 13071-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13071-1-PBS in a different storage buffer formulation.

产品信息

13071-1-PBS targets CD97 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD97 fusion protein Ag3745 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD97 molecule
Synonyms CD97 molecule, CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5
Calculated Molecular Weight 722 aa, 79 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC026690
Gene Symbol CD97
Gene ID (NCBI) 976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48960
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).