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验证数据展示

  • WB analysis using 84255-6-RR (same clone as 84255-6-PBS)

    WB analysis using 84255-6-RR (same clone as 84255-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84255-6-RR (CD97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84255-6-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84255-6-RR (same clone as 84255-6-PBS)

    IHC staining of human tonsillitis using 84255-6-RR (same clone as 84255-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84255-6-RR (CD97 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84255-6-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 84255-6-RR (same clone as 84255-6-PBS)

    IF Staining of Jurkat using 84255-6-RR (same clone as 84255-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (84255-6-RR, Clone: 241335A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84255-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84255-6-RR

    Affinity and Kinetic Characterization of 84255-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84255-6-RR against Human CD97 were performed. The affinity constant is 2.73 nM.

CD97 Recombinant antibody, PBS Only

CD97 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84255-6-PBS

产品说明书

CloneNo. 241335A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5, ADGRE5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84255-6-PBS targets CD97 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD97 molecule
别名 CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5, ADGRE5
计算分子量 722 aa, 79 kDa
观测分子量75 kDa
GenBank蛋白编号BC026690
基因名称 CD97
Gene ID (NCBI) 976
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP48960
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).