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CD97 Recombinant antibody, PBS Only

CD97 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241335A11

Cat no : 84255-6-PBS

Print datasheet

Synonyms

CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5, ADGRE5

验证数据展示

  • WB analysis using 84255-6-RR (same clone as 84255-6-PBS)

    WB analysis using 84255-6-RR (same clone as 84255-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84255-6-RR (CD97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84255-6-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84255-6-RR (same clone as 84255-6-PBS)

    IHC staining of human tonsillitis using 84255-6-RR (same clone as 84255-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84255-6-RR (CD97 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84255-6-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 84255-6-RR (same clone as 84255-6-PBS)

    IF Staining of Jurkat using 84255-6-RR (same clone as 84255-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (84255-6-RR, Clone: 241335A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84255-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84255-6-RR

    Affinity and Kinetic Characterization of 84255-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84255-6-RR against Human CD97 were performed. The affinity constant is 2.73 nM.

产品信息

84255-6-PBS targets CD97 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD97 molecule
Synonyms CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5, ADGRE5
Calculated Molecular Weight 722 aa, 79 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC026690
Gene Symbol CD97
Gene ID (NCBI) 976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP48960
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).