CD99 Polyclonal antibody

CD99 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 23079-1-AP

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Synonyms

12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2



经过测试的应用

Positive WB detected inJurkat cells, U-937 cells, THP-1 cells
Positive IHC detected inhuman pancreas tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23079-1-AP targets CD99 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF, IHC
Tested Reactivity human
Cited Reactivityhuman, pig
Immunogen CD99 fusion protein Ag19461 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD99 molecule
Synonyms 12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2
Calculated Molecular Weight 19 kDa, 16 kDa, 17 kDa
Observed Molecular Weight 28 kDa, 32 kDa
GenBank Accession NumberBC021620
Gene Symbol CD99
Gene ID (NCBI) 4267
RRIDAB_2879205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP14209
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).

实验方案

Product Specific Protocols
WB protocol for CD99 antibody 23079-1-APDownload protocol
IHC protocol for CD99 antibody 23079-1-APDownload protocol
FC protocol for CD99 antibody 23079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigIHC,IF

J Anim Sci Biotechnol

Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis.

Authors - Lingkai Zhang
humanIHC

Oncol Lett

Metastatic extraskeletal Ewing's sarcoma treated with trabectedin: A case report.

Authors - Jorge Hernando-Cubero
humanIHC

Cell Death Dis

KDM5B promotes tumorigenesis of Ewing sarcoma via FBXW7/CCNE1 axis.

Authors - Binbin Chen